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psm1 ncad egfp pp  (Addgene inc)


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    Structured Review

    Addgene inc psm1 ncad egfp pp
    Psm1 Ncad Egfp Pp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+lifeact+mruby2+blastr/pLenti+Lifeact-mRuby2+BlastR+(Plasmid+%2384384)/pm39905109-235-29-44
    Average 93 stars, based on 9 article reviews
    psm1 ncad egfp pp - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Aberrant activation of Rho/ROCK signaling in impaired polarity switching of colorectal micropapillary carcinoma.
    Article Snippet: Micropapillary carcinoma (MPC) is a morphologically distinctive form of carcinoma, composed of small nests of cancer cells surrounded by lacunar spaces.. Invasive MPC is associated with poor prognosis.. The nests of tumor cells in MPC reportedly exhibit reverse polarity, although the molecular mechanisms underlying MPC patterns are poorly understood.

    Article Title: Cadherin-based adhesion regulates mechanical polarization in the actin cortex through Rac1
    Article Snippet: The following day, the cultures were washed394 once with warmed Opti-MEM (Gibco) and left in 7 ml of the same medium containing 2%395 heat-inactivated FBS. .. The dishes were returned to incubator until transfection procedures.396 The plasmids were combined into a sterile 2 ml low DNA binding microtube (Sarstedt)397 containing Opti-MEM up to a final volume of 720 μl as follows: 10.0 μg of pSM1-NCAD-398 EGFP-PP or pLenti-Lifeact-mRuby2 BlastR (84384, Addgene [80]); 6.67 μg of psPAX2 (gift399 from Didier Trono, 12260, Addgene), and 3.33 μg of pMD2.G (gift from Didier Trono, 12259,400 Addgene). ..

    Construct:

    Article Title: Aberrant activation of Rho/ROCK signaling in impaired polarity switching of colorectal micropapillary carcinoma.
    Article Snippet: Micropapillary carcinoma (MPC) is a morphologically distinctive form of carcinoma, composed of small nests of cancer cells surrounded by lacunar spaces.. Invasive MPC is associated with poor prognosis.. The nests of tumor cells in MPC reportedly exhibit reverse polarity, although the molecular mechanisms underlying MPC patterns are poorly understood.

    Expressing:

    Article Title: EVL and MIM/MTSS1 regulate actin cytoskeletal remodeling to promote dendritic filopodia in neurons
    Article Snippet: Validated Mus musculus shRNA sequences were obtained from The RNAi Consortium (The Broad Institute via MilliporeSigma; ) and oligos were ligated between the AgeI-EcoRI sites (replacing the 1.9 kb stuffer) of our pLKO.1 TurboRFP cloning vector using annealed oligo cloning. shRNA sequences and source MilliporeSigma product number are given in . .. Lentiviral LifeAct expression vectors were published previously ( ; ): pLenti LifeAct-EGFP BlastR (RRID:Addgene_84383), pLenti-LifeAct-mRuby2 BlastR (RRID:Addgene_84384), pLenti LifeAct-iRFP670 BlastR (RRID:Addgene_84385). .. Lentiviral cDNA expression vectors were generated by PCR and subcloning cDNA of interest into the transfer plasmids pLenti CMVie-IRES-BlastR or pLenti CMVie-IRES-BlastR alt MCS (pCIB) published previously ( ; RRID:Addgene_119863 and RRID:Addgene_120862).

    Article Title: EVL and MIM/MTSS1 regulate actin cytoskeletal remodeling to promote dendritic filopodia in developing neurons
    Article Snippet: Validated Mus musculus shRNA sequences were obtained from The RNAi Consortium (The Broad Institute via MilliporeSigma, ( )) and oligos were ligated between the AgeI-EcoRI sites (replacing the 1.9kb stuffer) of our pLKO.1 TurboRFP cloning vector using annealed oligo cloning. shRNA sequences and source MilliporeSigma product number are given in Key Resources Table. .. Lentiviral LifeAct expression vectors were published previously ( ; ): pLenti LifeAct-EGFP BlastR (Addgene #84383; RRID:Addgene_84383), pLenti-LifeAct-mRuby2 BlastR (Addgene #84384; RRID:Addgene_84384), pLenti LifeAct-iRFP670 BlastR (Addgene #84385; RRID:Addgene_84385). .. Lentiviral cDNA expression vectors were generated by PCR and subcloning cDNA of interest into the transfer plasmids pLenti CMVie-IRES-BlastR or pLenti CMVie-IRES-BlastR alt MCS (pCIB) published previously ( ) (Addgene #119863 and #120862; RRID:Addgene_119863 and RRID:Addgene_120862).

    Plasmid Preparation:

    Article Title: High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons
    Article Snippet: .. Lentiviral transfer plasmid pLenti Lifeact-mRuby2 BlastR was generated by Multisite Gateway recombination of pENTR CMVie-Lifeact-mRuby2 L1-R5 (Addgene #84389), pMuLE ENTR MCS L5-L2 (Addgene #62085), and pLenti Dest BlastR (Addgene #84574). pLenti TurboRFP BlastR and pLenti Lifeact-mRuby2 BlastR have been deposited to Addgene under #102343 and #84384, respectively. .. For exogenous protein expression in cultured neurons, second generation lentiviral particles were generated by PEI transfection of 293T cells as previously described ( ) with transfer plasmid, pMD2.G, and psPAX2 (Addgene #12259, #12260, gifts from Didier Trono).

    Article Title: High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons
    Article Snippet: .. Lentiviral transfer plasmid pLenti Lifeact-mRuby2 BlastR was generated by Multisite Gateway recombination of pENTR CMVieLifeact-mRuby2 L1-R5 (Addgene #84389), pMuLE ENTR MCS L5-L2 (Addgene #62085), and pLenti Dest BlastR (Addgene #84574). pLenti TurboRFP BlastR and pLenti Lifeact-mRuby2 BlastR have been deposited to Addgene under #102343 and #84384, respectively. .. For exogenous protein expression in cultured neurons, second generation lentiviral particles were generated by PEI transfection of 293T cells as previously described (Yang et al., 2017) with transfer plasmid, pMD2.G, and psPAX2 (Addgene #12259, #12260, gifts from Didier Trono).

    Generated:

    Article Title: High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons
    Article Snippet: .. Lentiviral transfer plasmid pLenti Lifeact-mRuby2 BlastR was generated by Multisite Gateway recombination of pENTR CMVie-Lifeact-mRuby2 L1-R5 (Addgene #84389), pMuLE ENTR MCS L5-L2 (Addgene #62085), and pLenti Dest BlastR (Addgene #84574). pLenti TurboRFP BlastR and pLenti Lifeact-mRuby2 BlastR have been deposited to Addgene under #102343 and #84384, respectively. .. For exogenous protein expression in cultured neurons, second generation lentiviral particles were generated by PEI transfection of 293T cells as previously described ( ) with transfer plasmid, pMD2.G, and psPAX2 (Addgene #12259, #12260, gifts from Didier Trono).

    Article Title: High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons
    Article Snippet: .. Lentiviral transfer plasmid pLenti Lifeact-mRuby2 BlastR was generated by Multisite Gateway recombination of pENTR CMVieLifeact-mRuby2 L1-R5 (Addgene #84389), pMuLE ENTR MCS L5-L2 (Addgene #62085), and pLenti Dest BlastR (Addgene #84574). pLenti TurboRFP BlastR and pLenti Lifeact-mRuby2 BlastR have been deposited to Addgene under #102343 and #84384, respectively. .. For exogenous protein expression in cultured neurons, second generation lentiviral particles were generated by PEI transfection of 293T cells as previously described (Yang et al., 2017) with transfer plasmid, pMD2.G, and psPAX2 (Addgene #12259, #12260, gifts from Didier Trono).

    Sterility:

    Article Title: N-Cadherin based adhesion and Rac1 activity regulate tension polarization in the actin cortex.
    Article Snippet: .. The plasmids were combined into a sterile 2 ml low DNA binding microtube (Sarstedt) containing Opti-MEM up to a final volume of 720 μl as follows: 10.0 μg of pSM1-NCAD-EGFP-PP or pLenti-Lifeact-mRuby2 BlastR (84384, Addgene93); 6.67 μg of psPAX2 (gift from Didier Trono, 12260, Addgene), and 3.33 μg of pMD2.G (gift from Didier Trono, 12259, Addgene). ..

    Article Title: N-Cadherin based adhesion and Rac1 activity regulate tension polarization in the actin cortex
    Article Snippet: The following day, the cultures were washed once with warmed Opti-MEM (Gibco) and left in 7 ml of the same medium containing 2% heat-inactivated FBS. .. The plasmids were combined into a sterile 2 ml low DNA binding microtube (Sarstedt) containing Opti-MEM up to a final volume of 720 μl as follows: 10.0 μg of pSM1-NCAD-EGFP-PP or pLenti-Lifeact-mRuby2 BlastR (84384, Addgene ); 6.67 μg of psPAX2 (gift from Didier Trono, 12260, Addgene), and 3.33 μg of pMD2.G (gift from Didier Trono, 12259, Addgene). .. The plasmids were combined into a sterile 2 ml low DNA binding microtube (Sarstedt) containing Opti-MEM up to a final volume of 720 μl as follows: 10.0 μg of pSM1-NCAD-EGFP-PP or pLenti-Lifeact-mRuby2 BlastR (84384, Addgene ); 6.67 μg of psPAX2 (gift from Didier Trono, 12260, Addgene), and 3.33 μg of pMD2.G (gift from Didier Trono, 12259, Addgene).

    Article Title: Cadherin-based adhesion regulates mechanical polarization in the actin cortex through Rac1
    Article Snippet: The following day, the cultures were washed394 once with warmed Opti-MEM (Gibco) and left in 7 ml of the same medium containing 2%395 heat-inactivated FBS. .. The dishes were returned to incubator until transfection procedures.396 The plasmids were combined into a sterile 2 ml low DNA binding microtube (Sarstedt)397 containing Opti-MEM up to a final volume of 720 μl as follows: 10.0 μg of pSM1-NCAD-398 EGFP-PP or pLenti-Lifeact-mRuby2 BlastR (84384, Addgene [80]); 6.67 μg of psPAX2 (gift399 from Didier Trono, 12260, Addgene), and 3.33 μg of pMD2.G (gift from Didier Trono, 12259,400 Addgene). ..

    Binding Assay:

    Article Title: N-Cadherin based adhesion and Rac1 activity regulate tension polarization in the actin cortex.
    Article Snippet: .. The plasmids were combined into a sterile 2 ml low DNA binding microtube (Sarstedt) containing Opti-MEM up to a final volume of 720 μl as follows: 10.0 μg of pSM1-NCAD-EGFP-PP or pLenti-Lifeact-mRuby2 BlastR (84384, Addgene93); 6.67 μg of psPAX2 (gift from Didier Trono, 12260, Addgene), and 3.33 μg of pMD2.G (gift from Didier Trono, 12259, Addgene). ..

    Article Title: N-Cadherin based adhesion and Rac1 activity regulate tension polarization in the actin cortex
    Article Snippet: The following day, the cultures were washed once with warmed Opti-MEM (Gibco) and left in 7 ml of the same medium containing 2% heat-inactivated FBS. .. The plasmids were combined into a sterile 2 ml low DNA binding microtube (Sarstedt) containing Opti-MEM up to a final volume of 720 μl as follows: 10.0 μg of pSM1-NCAD-EGFP-PP or pLenti-Lifeact-mRuby2 BlastR (84384, Addgene ); 6.67 μg of psPAX2 (gift from Didier Trono, 12260, Addgene), and 3.33 μg of pMD2.G (gift from Didier Trono, 12259, Addgene). .. The plasmids were combined into a sterile 2 ml low DNA binding microtube (Sarstedt) containing Opti-MEM up to a final volume of 720 μl as follows: 10.0 μg of pSM1-NCAD-EGFP-PP or pLenti-Lifeact-mRuby2 BlastR (84384, Addgene ); 6.67 μg of psPAX2 (gift from Didier Trono, 12260, Addgene), and 3.33 μg of pMD2.G (gift from Didier Trono, 12259, Addgene).

    Article Title: Cadherin-based adhesion regulates mechanical polarization in the actin cortex through Rac1
    Article Snippet: The following day, the cultures were washed394 once with warmed Opti-MEM (Gibco) and left in 7 ml of the same medium containing 2%395 heat-inactivated FBS. .. The dishes were returned to incubator until transfection procedures.396 The plasmids were combined into a sterile 2 ml low DNA binding microtube (Sarstedt)397 containing Opti-MEM up to a final volume of 720 μl as follows: 10.0 μg of pSM1-NCAD-398 EGFP-PP or pLenti-Lifeact-mRuby2 BlastR (84384, Addgene [80]); 6.67 μg of psPAX2 (gift399 from Didier Trono, 12260, Addgene), and 3.33 μg of pMD2.G (gift from Didier Trono, 12259,400 Addgene). ..



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